WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Review Journal for Medical Science and Pharma Professionals

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

World Journal of Advance Healthcare Research (WJAHR) has indexed with various reputed international bodies like : Google Scholar , Index Copernicus , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , Cosmos Impact Factor , Scientific Indexing Services (SIS) , UDLedge Science Citation Index , International Impact Factor Services , International Society for Research Activity (ISRA) Journal Impact Factor (JIF) , IFSIJ Measure of Journal Quality , Scientific Journal Impact Factor (SJIF) , International Scientific Indexing, UAE (ISI) (Under Process) , International Impact Factor Services (IIFS) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Academia , Scope Database , 

ISSN 2457-0400

Impact Factor  :  6.711

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    6th INTERNATIONAL CONFERENCE ON EDUCATIONAL TECHNOLOGY AND CURRICULUM STUDIES(ICETC2019) 

     

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Best Article of current issue

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Indexing

Abstract

PHYTOCHEMICAL SCREENING, DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR ISOLATED STIGMASTEROL FROM OCIMUM SANCTUM

Apporva Chawla*, Atinderpal Singh, Shaina Sharma, Varinder Soni

ABSTRACT

Stigmasterol is the abundant phytoconstituent present in the leaves of Ocimum sanctum, having a major function to maintain the structure and physiology of cell membranes. The dried leaves of Ocimum sanctum was subjected to extraction, preliminary phytochemical screening and then isolation. The identification of the isolated compound was confirmed by TLC, HPLC profile and spectral studies by comparing with the standard. RP-HPLC method were developed and validated as per ICH guidelines using the isolated biomarker compounds such as stigmasterol. Results of HPLC analysis of isolated compound shows the presence of stigmasterol which is confirmed by the single peaks present in the chromatogram at 3.18 retention time (in mins). The reported data may be of great value as a reference standard for evaluation of the isolated compound.

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