WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

Obeagu Emmanuel Ifeanyi*, Vincent C.C.N. and Anaebo Queen Braxton N.

ABSTRACT

Diabetes mellitus especially the type 2 is a serious global public health threat to the existence of human beings on earth. The study was done to determine the levels of erythropoietin and red cell parameters in patients with type 2 diabetes mellitus in Southeast, Nigeria. The study was done in a tertiary health institution. A total of 200 subjects were recruited for the study comprising 100 subjects each for Patients with type 2 DM (50 subjects were Males, 50 were Females) and 100 subjects for apparently healthy subjects (Control) (50 subjects were Males, 50 were Females) drawn from the Health institution. About 6ml of venous blood was aseptically collected from the antecubital vein of each subject by standard technique. About 4.5ml was dispensed into plain tubes for Erythropoietin assay and the remaining was dispensed into an EDTA bottle for haematological parameters determination. All reagents and kits were commercially purchased from reputable company whose standard operating procedures were strictly followed. Human EPO (Erythropoietin) ELISA kit was purchased from Elabscience with catalog No: E-EL-H0066c. The haematological parameters were determined using Mindray BC-5300. The results were expressed as mean± standard deviation. The data were analysed with the statistical package for social science (SPSS) version 21 using t-test, ANOVA and the level of significance was set at P

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