WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Review Journal for Medical Science and Pharma Professionals

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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  • Google Scholar
  • Index Copernicus
  • SOCOLAR, China
  • Research Bible, Fuchu, Tokyo. JAPAN
  • Cosmos Impact Factor
  • Scientific Indexing Services (SIS)
  • UDLedge Science Citation Index
  • International Impact Factor Services
  • International Society for Research Activity (ISRA) Journal Impact Factor (JIF)
  • Scientific Journal Impact Factor (SJIF)
  • IFSIJ Measure of Journal Quality
  • International Scientific Indexing, UAE (ISI) (Under Process)
  • International Impact Factor Services (IIFS)
  • Web of Science Group (Under Process)
  • Directory of Research Journals Indexing
  • Scholar Article Journal Index (SAJI)
  • International Scientific Indexing ( ISI )
  • Academia
  • Scope Database
  • Research Publication Rating and Indexing
  • Doi-Digital Online Identifier
  • ISSN National Centre
  • Zenodo Indexing
  • International CODEN Service, USA

Abstract

DETERMINE THE BEST CONDITIONS FOR EXTRACTING BIOATIVE COMPOUNDS FROM ROSMARINUS OFFICINALIS LEAVES

*Eman Al-Mnsour

ABSTRACT

The aim of this study is to study the effect of some factors on the efficiency of ultrasound assisted extraction of some compounds from Rosmarinus officinalis in order to determine the best extraction conditions, detection of Polyphenol compounds in extracts, and test the biological activity of alcoholic and water extracts of leaves of rosemary (Rosmarinus officinalis) against some Gram- negative pathogens (Escherichia. coli) and Gram-positive (Staphylococcus aureus) by agar well diffusion method. The results of extracts showed that the best conditions are: the solvent is ethyl alcohol 85%, temperature is 45 0C, Time is 40 min. alcoholic extract gave a higher extraction yield and Polyphenol content, and more biological activity. the highest biological activity was at concentration (100mg/ml), of alcoholic extract, the average inhibition diameter was 19 mm against Escherichia. coli, and 15 mm against Staphylococcus aureus, while the aqueous extract did not show any activity against the bacteria under study until the concentration 100 mg/ml.

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