WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

Ganesh Akula*, Bandoj Nikhitha, Manumula Jusya Vani, Jonnada Swetha, Saniya Begum, Joganolla Sri Vidya

ABSTRACT

To determine the content of caffeine in the beverages like green tea, black tea, and soft drink which are commercially available in the local market with the help of a UV-Visible spectrophotometer. Chloroform was used as the solvent and concentrations of caffeine were measured at the wavelength of 274 nm. Using chloroform as an extractant, the caffeine was extracted from them, and the UV-Visible spectrophotometer was used to measure it both quantitatively and qualitatively. Caffeine, a naturally occurring stimulant, is widely consumed worldwide in various forms such as tea, coffee, and soft drinks. It plays a significant role in human daily life, impacting cognitive functions, alertness, and metabolic activities. Thestandardsolutions of caffeine from the range 2-30 ppm were prepared in the chloroform, show the linearity with the correlation coefficient of 0.99. From the calibration curve, the concentration of caffeine was determined in various brands. It is observed that black tea contains the maximum caffeine content followed by green tea and soft drinks. In this study the maximum caffeine content find out was (45.6mg/g)in the Nice black tea sample and the minimum (0.161mg/ml) in Pepsi soft drink sample. The caffeine content reported here is higher than that of previous studies may be due to the modified approach treatment temperature (90-100ºC) and longer brewing time (5 minutes) in this study. The use of the UVVisible spectrophotometer is also an alternative to the HPLC technique, thereby the working cost could be brought down.

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