WORLD JOURNAL OF ADVANCE
HEALTHCARE RESEARCH

*Mohammed Hassan Muzher Al. Mayahi and Proof. Dr. Maitham Ghaly Yousif

ABSTRACT

Objective: To isolate and identify E. coli strains from samples taken from patients with gut disorders, analyze the genetic diversity among isolates using RAPD-PCR to detect genetic variation among them, To assess the genetic relationship between isolates to determine their genetic closeness or divergence, and to correlate this relationship with the clinical condition of patients. Methods: Samples were collected from Diwaniyah Teaching Hospital, Digestive System Unit. Twenty E. coli bacteria were isolated from 35 samples from patients suffering from intestinal disorders accompanied by watery and bloody diarrhoea, from both sexes, males and females, using agricultural biochemical methods and genetic diversity using RAPD PCR and branching tree analysis. Results: 20 isolates of E. coli bacteria were selected from patients suffering from gut disorders to determine their genetic heterogeneity using ERIC-PCR. and shows the presence of polymorphic variants within each of the six groups. The number of polymorphic variants (genetic variants) among isolates can be estimated at approximately 8. Conclusion: in this study, males were more likely than females to be affected by gut disorders. The age group most affected by gut disorders and the highest rate of isolation of E. coli bacteria was from 21-30 years. E. coli bacteria also had genetic diversity with six clusters and eight genetic variations.