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TUMOR INHIBITORY L-ASPARAGINASE PRODUCTION
Praveen Kumar Dasari*, Karri Triveni, Thopula Poojitha, Thota Divya, Yadamakanti Sandhya Rani, Maddela Nikhil and Ummadi Bhavani
The present research work demonstrates the production of L-asparaginase using Neolamarckia cadamba as substrate by Streptomyces ambofaciens in a solid state fermentation. Generally L-Asparaginase enzyme was present in many animal tissues, plant tissues and bacterial cell, but not in human cell. L-asparaginase is a chemotherapeutic agent plays a vital role in treatment of lymphosarcoma, lymphoproliferative disorders and acute lymphocytic leukemia. In this research, L-asparaginase enzyme production parameters like incubation time, incubation temperature, pH, inoculum level and moisture content were optimized. Different carbon supplements were screened for their influence on enzyme activity; they are sucrose, maltose, glucose, fructose, and lactose. The fermentation time of 48hrs, the temperature of 320C, pH 6.0, inoculum level of 80%v/w and moisture content of 70%v/w were observed optimum for the production of L-asparginase. Among carbon supplements sucrose gave better yield. L-asparagine of 0.3% w/w as nitrogen source was observed optimum for the production of L-asparaginase. Final conclusion that Neolamarckia cadamba could be a promising agent for industrial application since it produces a significant L–asparaginase (85.66 U/ml) activity in solid state fermentation.[Full Text Article]